Shiny Error - Seurat Integration | Reactive Value error

shiny
1 
seuratObj <- reactive ({
    CreateSeuratObject(exp.data, min.cells = input$minCells, min.genes = input$minGenes, project = input$project)
  })
  #so <- seuratObj()
  mito.genes <- grep(pattern = "^MT-", x = rownames(x = seuratObj()@data), value = TRUE)
   
  percent.mito <- Matrix::colSums(seuratObj()@raw.data[mito.genes, ])/Matrix::colSums(seuratObj()@raw.data)

  
  
  seuratObj2 <- reactive({
    AddMetaData(object = seuratObj(), metadata = percent.mito, col.name = "percent.mito")
  })    
  
  output$plot <- renderPlot({
    VlnPlot(object = seuratObj2(), features.plot = c("nGene","nUMI",  "percent.mito", ncol = 3))
  })
2

What is the error that you are getting? You will get more helpful suggestions if you follow the guidelines in the Shiny debugging and reprex guide.

2 Likes
3

bad file argument after the app is launched.