Creating a subset variation data

I need to create a subset of the variation data, I run the code and the original data is there but when I get to the subset it shows up as 0 obs. of 5 variables . I'm not sure what's wrong with my code.

^this is how it shows up after I run it
(here is the code used)
variationdatatemplate <- read_csv("variationdatatemplate.csv")
variationdata <- read.csv("variationdatatemplate.csv")
Euglenadata <- subset(variationdata, Group=="Euglena1" | Group=="Euglena2")
GreylingData <- subset(variationdata, Group=="Greyling1" | Group=="Greyling2")


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