If I had to guess, the issue here is because you have Windows packages specified and shinyapps is a linux machine:
pywin32==305.1
pywin32-ctypes
To specify the minimum requirements for your app, you can use something like pipreqs, which will scan your actual code for what you are importing and just specify those dependencies. This can often resolve the issue I describe with having OS-specific dependencies that you aren't really necessary.
Best,
Randy